Efficiency of Human Epiphyseal Chondrocytes with Differential Replication Numbers for Cellular Therapy Products
نویسندگان
چکیده
منابع مشابه
Efficiency of Human Epiphyseal Chondrocytes with Differential Replication Numbers for Cellular Therapy Products
The cell-based therapy for cartilage or bone requires a large number of cells; serial passages of chondrocytes are, therefore, needed. However, fates of expanded chondrocytes from extra fingers remain unclarified. The chondrocytes from human epiphyses morphologically changed from small polygonal cells to bipolar elongated spindle cells and to large polygonal cells with degeneration at early pas...
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We have observed that epiphyseal and physeal hypertrophic chondrocytes in BALB/c mice show considerable differences of light microscopic and ultrastructural appearance, even when the cells are at the same stage of differentiation. In addition, cell structure maintenance improved with tissue preparation controlled for osmolarity and for membrane stabilization using 0.5% ruthenium hexammine trich...
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Epiphyseal chondrocytes must exist in demanding microenvironmental conditions, facing continuous low oxygen tensions and high lactic acid concentrations. This requires chondrocytes to generate their energy to a significant degree through anaerobic glycolysis. In this pathway, one molecule of glucose is metabolized into two molecules of pyruvate and two molecules of ATP. Under normoxic condition...
متن کاملHigh Quality of Infant Chondrocytes in Comparison with Adult Chondrocytes for Cartilage Tissue Engineering
BACKGROUND Tissue engineering is used for the treatment of many diseases, and the ideal cell source for cartilage tissue engineering is chondrocytes. The main limitation of chondrocyte is the low number of cells in cartilage tissue engineering. This study investigated a suitable cell source with high proliferation rate to obtain a large number of chondrocytes. METHODS Adult cartilage t...
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ژورنال
عنوان ژورنال: BioMed Research International
سال: 2016
ISSN: 2314-6133,2314-6141
DOI: 10.1155/2016/6437658